6OVS

Coiled-coil Trimer with Glu:Tyr:Lys Triad

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	ENRAF-NONIUS FR591
Temperature [K]	100
Detector technology	CCD
Collection date	2017-08-26
Detector	APEX II CCD
Wavelength(s)	1.5406
Spacegroup name	H 3
Unit cell lengths	39.266, 39.266, 99.823
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	16.910 - 1.800
R-factor	0.149
Rwork	0.146
R-free	0.17370
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	PROTEUM PLUS
Data scaling software	PROTEUM PLUS
Phasing software	PHENIX
Refinement software	PHENIX (dev_2906)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	16.910	1.865
High resolution limit [Å]	1.800	1.800
Rmerge	0.099	0.318
Rmeas	0.110	0.389
Rpim	0.045	0.209
Number of reflections	5310	525
<I/σ(I)>	12.7	1.4
Completeness [%]	99.8
Redundancy	4.7
CC(1/2)	0.991	0.848

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	30% PEG600, 10% glycerol, 100 mM HEPES-NaOH, pH 7.5, 50 mM lithium sulfate