6OVS
Coiled-coil Trimer with Glu:Tyr:Lys Triad
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | ENRAF-NONIUS FR591 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-08-26 |
Detector | APEX II CCD |
Wavelength(s) | 1.5406 |
Spacegroup name | H 3 |
Unit cell lengths | 39.266, 39.266, 99.823 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 16.910 - 1.800 |
R-factor | 0.149 |
Rwork | 0.146 |
R-free | 0.17370 |
Structure solution method | MOLECULAR REPLACEMENT |
Data reduction software | PROTEUM PLUS |
Data scaling software | PROTEUM PLUS |
Phasing software | PHENIX |
Refinement software | PHENIX (dev_2906) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 16.910 | 1.865 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.099 | 0.318 |
Rmeas | 0.110 | 0.389 |
Rpim | 0.045 | 0.209 |
Number of reflections | 5310 | 525 |
<I/σ(I)> | 12.7 | 1.4 |
Completeness [%] | 99.8 | |
Redundancy | 4.7 | |
CC(1/2) | 0.991 | 0.848 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 298 | 30% PEG600, 10% glycerol, 100 mM HEPES-NaOH, pH 7.5, 50 mM lithium sulfate |