6OVP
Sterol Carrier Protein 2 from Yarrowia Lipolytica (apo form)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-02-17 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.9801 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 67.920, 67.920, 117.040 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 41.486 - 1.991 |
| R-factor | 0.2433 |
| Rwork | 0.241 |
| R-free | 0.29320 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB 4JGX |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.355 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.490 | 2.110 |
| High resolution limit [Å] | 1.990 | 1.990 |
| Rmeas | 0.054 | 2.585 |
| Number of reflections | 21964 | 3422 |
| <I/σ(I)> | 27.9 | 1 |
| Completeness [%] | 99.7 | 98.4 |
| Redundancy | 19.7 | 20.1 |
| CC(1/2) | 1.000 | 0.570 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 294 | HEPES 0.1 M, sodium citrate 1.4 M, pH 6.5-7.0 |
