6OVL
2.1 Angstrom structure of wild type Glyoxylate/Hydroxypyruvate reductase A from Escherichia Coli in complex with glyoxylate and NADP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-03-18 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97856 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 158.820, 158.820, 96.710 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 36.730 - 2.100 |
R-factor | 0.1646 |
Rwork | 0.164 |
R-free | 0.18380 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3kbo |
RMSD bond length | 0.003 |
RMSD bond angle | 0.698 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.11.1_2575)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 36.730 | 2.175 |
High resolution limit [Å] | 2.100 | 2.100 |
Number of reflections | 41974 | 3924 |
<I/σ(I)> | 26.2 | |
Completeness [%] | 99.1 | |
Redundancy | 16.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 298 | 1.4 M sodium phosphate monobasic/ potassium phosphate dibasic, 5 mM NADP, 100 mM sodium glyoxylate |