6OV6

CRYSTALLOGRAPHIC STRUCTURE OF THE C24 PROTEIN FROM THE ANTARCTIC MICROORGANISM BIZIONIA ARGENTINENSIS

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-11-17
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.98011
Spacegroup name	P 21 21 21
Unit cell lengths	63.130, 65.250, 239.530
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.120 - 1.820
R-factor	0.199
Rwork	0.198
R-free	0.23000
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	1.100
Data reduction software	XDS
Data scaling software	XDS
Phasing software	SHELX
Refinement software	BUSTER (2.10.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.930
High resolution limit [Å]	1.820	1.820
Rmerge	0.053	0.593
Number of reflections	87380	6246
<I/σ(I)>	17.3	2.4
Completeness [%]	97.2	95.6
Redundancy	5.6	5.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	295	12% PEG 8000, 0.1 M SODIUM CITRATE, 0.1 M AMMONIUM SULFATE
1	VAPOR DIFFUSION, HANGING DROP	7.5	295	12% PEG 8000, 0.1 M SODIUM CITRATE, 0.1 M AMMONIUM SULFATE