6OV1
Structure of Staphylococcus aureus RNase P protein mutant with defective mRNA degradation activity
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2017-07-31 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.0322 |
Spacegroup name | P 1 |
Unit cell lengths | 40.386, 43.467, 45.837 |
Unit cell angles | 91.11, 114.83, 110.95 |
Refinement procedure
Resolution | 40.761 - 1.660 |
R-factor | 0.1905 |
Rwork | 0.187 |
R-free | 0.23840 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1d6t |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.720 |
High resolution limit [Å] | 1.657 | 3.580 | 1.660 |
Rmerge | 0.042 | 0.027 | 0.513 |
Rmeas | 0.059 | 0.038 | 0.725 |
Rpim | 0.042 | 0.027 | 0.513 |
Number of reflections | 29214 | 2983 | 2838 |
<I/σ(I)> | 6.8 | ||
Completeness [%] | 95.2 | 97.1 | 92.2 |
Redundancy | 1.8 | 1.8 | 1.8 |
CC(1/2) | 0.996 | 0.573 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298.15 | 0.1 M Imidazole-HCl, pH8 1.3 M Sodium Citrate |