6OU2
Crystal Structure of the D478N Variant of the Myocilin Olfactomedin Domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2016-03-09 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 44.969, 57.250, 90.356 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.259 - 1.963 |
| R-factor | 0.1693 |
| Rwork | 0.166 |
| R-free | 0.19760 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.660 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.260 | 2.030 |
| High resolution limit [Å] | 1.960 | 1.960 |
| Rmerge | 0.132 | |
| Number of reflections | 17202 | 1633 |
| <I/σ(I)> | 16.7 | 4.8 |
| Completeness [%] | 99.6 | |
| Redundancy | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 20% PEG 3350, 0.2M Bis Tris pH 6.0, 0.3 M Magnesium formate |
