6OTN

Crystal Structure of an N-terminal Fragment of Cancer Associated Tropomyosin 3.1 (Tpm3.1)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	90
Detector technology	PIXEL
Collection date	2018-08-16
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.979338
Spacegroup name	P 43 21 2
Unit cell lengths	97.201, 97.201, 93.100
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.560 - 2.400
R-factor	0.238
Rwork	0.235
R-free	0.26580
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ic2
Data reduction software	XDS
Data scaling software	Aimless (0.5.21)
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.560	29.560	2.460
High resolution limit [Å]	2.400	10.720	2.400
Rmerge	0.084	0.039	0.791
Rmeas	0.086	0.040	0.819
Rpim	0.022	0.011	0.207
Total number of observations	274283	3120	17237
Number of reflections	17984	242	1196
<I/σ(I)>	23.2	58.6	3.4
Completeness [%]	99.3	95.3	90.9
Redundancy	15.3	12.9	14.4
CC(1/2)	0.999	0.999	0.911

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	Crystals were grown in hanging drops by mixing purified N_82AA_Tpm3.1 at 20 mg/ml with an equal volume of reservoir solution (0.1 M BisTris pH 6.5, 0.2 M Li2SO4, 12% polyethylene glycol 3350)