6OSR

Crystal structure of Influenza hemagglutinin from strain A/Melbourne/1/1946(H1N1)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2019-03-21
Detector	RAYONIX MX-225
Wavelength(s)	0.97872
Spacegroup name	P 1 21 1
Unit cell lengths	73.100, 113.970, 246.380
Unit cell angles	90.00, 91.44, 90.00

Refinement procedure

Resolution	48.736 - 2.550
R-factor	0.1921
Rwork	0.191
R-free	0.24590
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6mya
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX (dev_3409)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.736	48.736	2.620
High resolution limit [Å]	2.550	11.400	2.550
Rmerge	0.168	0.055	1.089
Rmeas	0.186	0.063	1.210
Total number of observations	691488
Number of reflections	131565	1513	9715
<I/σ(I)>	7.78	19.52	1.52
Completeness [%]	99.8	96.9	99.7
Redundancy	5.256	4.683	5.284
CC(1/2)	0.992	0.997	0.548

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	287	0.1 uL 10.1 mg/mL SEC-purified InvbX.18715.a.KN11.PD38349 in 25 mM Tris, pH 8.5, 150 mM sodium chloride + 0.1 uL mother liquor (0.2 M potassium citrate, 20% PEG3350, 3% 1,5-pentanediol), cryoprotection: mother liquor + 20% ethylene glycol, Crystal ID 308017b5, data set yxj7-1