6OS4
Calmodulin in complex with farnesyl cysteine methyl ester
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-06-17 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 1, 1.77 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 40.379, 40.379, 338.137 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 34.969 - 2.050 |
R-factor | 0.1945 |
Rwork | 0.191 |
R-free | 0.22490 |
Structure solution method | SAD |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.830 |
High resolution limit [Å] | 1.800 | 4.880 | 1.800 |
Rmerge | 0.049 | 0.035 | 0.480 |
Rmeas | 0.051 | 0.037 | 0.498 |
Rpim | 0.013 | 0.010 | 0.127 |
Total number of observations | 226154 | ||
Number of reflections | 16439 | 1047 | 789 |
<I/σ(I)> | 8.9 | ||
Completeness [%] | 99.6 | 100 | 94.4 |
Redundancy | 13.8 | 14.1 | 13 |
CC(1/2) | 0.999 | 0.969 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.7 | 293 | 0.2mM sodium acetate, 0.1mM cacodylate, 28% PEG 8000 |