6ORK
Crystal structure of Sel1 repeat protein from Oxalobacter formigenes
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-08-23 |
Detector | DECTRIS PILATUS3 X 6M |
Wavelength(s) | 0.9794 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 61.926, 103.368, 122.709 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.585 - 3.000 |
R-factor | 0.2114 |
Rwork | 0.209 |
R-free | 0.25380 |
Structure solution method | SAD |
Data reduction software | HKL-3000 |
Data scaling software | SCALEPACK |
Phasing software | HKL-3000 |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 3.050 |
High resolution limit [Å] | 3.000 | 8.130 | 3.000 |
Rmerge | 0.087 | 0.034 | 0.893 |
Rmeas | 0.096 | 0.037 | 1.012 |
Rpim | 0.039 | 0.016 | 0.461 |
Total number of observations | 93909 | ||
Number of reflections | 16196 | 922 | 663 |
<I/σ(I)> | 6.7 | ||
Completeness [%] | 98.1 | 99.5 | 83.2 |
Redundancy | 5.8 | 5.7 | 3.8 |
CC(1/2) | 0.998 | 0.598 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 289 | 10 mM tri-sodium citrate, 33% PEG6000 |