6ORH
Crystal structure of SpGH29
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-07-02 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97945 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 69.828, 98.429, 79.519 |
| Unit cell angles | 90.00, 97.26, 90.00 |
Refinement procedure
| Resolution | 27.840 - 1.620 |
| R-factor | 0.1715 |
| Rwork | 0.170 |
| R-free | 0.20490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3eyp |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.423 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.840 | 1.710 |
| High resolution limit [Å] | 1.620 | 1.620 |
| Rmerge | 0.050 | 0.337 |
| Rpim | 0.026 | 0.183 |
| Number of reflections | 131486 | 19408 |
| <I/σ(I)> | 17 | 4.7 |
| Completeness [%] | 97.2 | 98.4 |
| Redundancy | 4.5 | 4.5 |
| CC(1/2) | 0.998 | 0.882 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291 | 21 % PEG 4000, 0.22 M NaOAc, 1 mM DTT and 0.1 M Tris. |
