6ORD

Crystal structure of tRNA^ Ala(GGC) U32-A38 bound to cognate 70S A site

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-06-18
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97920
Spacegroup name	P 21 21 21
Unit cell lengths	209.380, 445.830, 616.030
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.998 - 3.100
R-factor	0.2331
Rwork	0.231
R-free	0.26820
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4y4o
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.15_3459)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	49.998	49.998	3.080
High resolution limit [Å]	3.000	13.420	3.000
Rmerge	0.201	0.035	0.739
Rmeas	0.240	0.040	0.986
Total number of observations	3052189
Number of reflections	1037562	12449	44061
<I/σ(I)>	4.35	19.09	0.56
Completeness [%]	91.3	91.9	52.6
Redundancy	2.942	3.953	1.471
CC(1/2)	0.991	0.999	0.194

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	0.1M L-arginine HCl, 0.1M Tris-HCl pH 7.5, 3% PEG 20K, 10-16.5% MPD (2-methyl-2,4-pentanediol), 1mM BME