6ORC
Crystal structure of Sel1 repeat protein from Oxalobacter formigenes
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-06-28 |
Detector | DECTRIS PILATUS3 X 6M |
Wavelength(s) | 0.9792 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 73.301, 75.479, 78.344 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.662 - 2.980 |
R-factor | 0.2101 |
Rwork | 0.208 |
R-free | 0.24760 |
Structure solution method | SAD |
Data reduction software | HKL-3000 |
Data scaling software | SCALEPACK |
Phasing software | HKL-3000 |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 3.020 |
High resolution limit [Å] | 2.970 | 8.050 | 2.970 |
Rmerge | 0.112 | 0.101 | 0.959 |
Rmeas | 0.125 | 0.114 | 1.085 |
Rpim | 0.053 | 0.051 | 0.496 |
Total number of observations | 51377 | ||
Number of reflections | 9035 | 503 | 422 |
<I/σ(I)> | 11.7 | ||
Completeness [%] | 99.4 | 96.2 | 98.6 |
Redundancy | 5.7 | 5.1 | 4.4 |
CC(1/2) | 0.939 | 0.641 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 289 | 0.1M Tris-HCl, 20 % PEG1000 |