6OR7

Structure of HIV-1 Reverse Transcriptase (RT) in complex with DNA AND (-)FTC-TP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-08-16
Detector	ADSC HF-4M
Wavelength(s)	0.9792
Spacegroup name	C 2 2 21
Unit cell lengths	166.499, 169.825, 103.283
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.572 - 2.530
R-factor	0.2138
Rwork	0.212
R-free	0.24930
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3kjv
RMSD bond length	0.003
RMSD bond angle	0.569
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.680
High resolution limit [Å]	2.530	2.530
Number of reflections	48991	7702
<I/σ(I)>	16.64	1.93
Completeness [%]	99.7	98.4
Redundancy	7.3	6.98
CC(1/2)	0.998	0.778

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	295	6-10% (w/v) PEG 8000, 15 mM magnesium sulfate, and 50 mM MES adjusted at pH 6.0