6OR7
Structure of HIV-1 Reverse Transcriptase (RT) in complex with DNA AND (-)FTC-TP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-08-16 |
| Detector | ADSC HF-4M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 166.499, 169.825, 103.283 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.572 - 2.530 |
| R-factor | 0.2138 |
| Rwork | 0.212 |
| R-free | 0.24930 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3kjv |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.569 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.680 |
| High resolution limit [Å] | 2.530 | 2.530 |
| Number of reflections | 48991 | 7702 |
| <I/σ(I)> | 16.64 | 1.93 |
| Completeness [%] | 99.7 | 98.4 |
| Redundancy | 7.3 | 6.98 |
| CC(1/2) | 0.998 | 0.778 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 295 | 6-10% (w/v) PEG 8000, 15 mM magnesium sulfate, and 50 mM MES adjusted at pH 6.0 |
