6OR4
Crystal structure of SpGH29
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-05-28 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 |
| Unit cell lengths | 49.960, 68.820, 72.670 |
| Unit cell angles | 76.58, 73.24, 73.61 |
Refinement procedure
| Resolution | 46.590 - 2.100 |
| R-factor | 0.1721 |
| Rwork | 0.169 |
| R-free | 0.23190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3eyp |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.371 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.590 | 2.160 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.062 | 0.344 |
| Rpim | 0.062 | 0.344 |
| Number of reflections | 50203 | 4093 |
| <I/σ(I)> | 14.1 | 3.5 |
| Completeness [%] | 98.0 | 96.9 |
| Redundancy | 3.9 | 4 |
| CC(1/2) | 0.998 | 0.895 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291 | 20 % PEG 3350, 0.2 M NaCl, 1 mM DTT and 0.1 M Tris. |
