6OR1

Human LRH-1 bound to the agonist 2N and a fragment of the Tif2 coregulator

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	80
Detector technology	PIXEL
Collection date	2018-03-17
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1
Spacegroup name	P 43 21 2
Unit cell lengths	46.658, 46.658, 222.696
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.033 - 2.174
R-factor	0.2017
Rwork	0.200
R-free	0.23870
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB 5L11
RMSD bond length	0.002
RMSD bond angle	0.422
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.280
High resolution limit [Å]	2.174	4.740	2.174
Rmerge	0.163	0.117
Rmeas	0.167	0.119
Rpim	0.036	0.026	0.309
Total number of observations	280833
Number of reflections	13301	1556	1170
<I/σ(I)>	3.4
Completeness [%]	96.6	99.6	87.9
Redundancy	21.1	20.4	13
CC(1/2)		0.996	0.697

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.05M sodium acetate (pH 4.6), 5-11% PEG4000, 0-10% glycerol. Microseeding with LRH-1-RJW100 crystals.