6OQ1
Crystal Structure of Branched K11/K48-Linked Tri-Ubiquitin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-08-09 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97921 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.810, 44.780, 115.390 |
| Unit cell angles | 90.00, 90.21, 90.00 |
Refinement procedure
| Resolution | 57.690 - 2.200 |
| R-factor | 0.2139 |
| Rwork | 0.213 |
| R-free | 0.22970 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ubq |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.652 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless (0.5.25) |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.690 | 2.270 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.180 | |
| Rmeas | 0.206 | |
| Rpim | 0.098 | |
| Total number of observations | 95721 | |
| Number of reflections | 22008 | 1881 |
| <I/σ(I)> | 5.3 | |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 4.3 | 4.4 |
| CC(1/2) | 0.987 | 0.265 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 295 | bis-tris, sodium chloride, PEG 3350 |
