6OPE

Crystal structure of tRNA^ Ala(GGC) U32-A38 bound to near-cognate 70S A site

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-04-23
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97910
Spacegroup name	P 21 21 21
Unit cell lengths	209.380, 449.760, 619.920
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.736 - 3.100
R-factor	0.2271
Rwork	0.225
R-free	0.26630
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4y4o
Data reduction software	XFIT
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.15_3459)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	49.736	49.736	3.180
High resolution limit [Å]	3.100	13.860	3.100
Rmerge	0.290	0.040	1.765
Rmeas	0.321	0.044	1.956
Total number of observations	5456813
Number of reflections	1016574	10798	76404
<I/σ(I)>	6.19	26.72	1.13
Completeness [%]	97.1	86.6	99.2
Redundancy	5.368	5.25	5.236
CC(1/2)	0.991	0.999	0.365

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	0.1M L-arginine HCl, 0.1M Tris-HCl pH 7.5, 3% PEG 20K, 10-16.5% MPD (2-methyl-2,4-pentanediol), 1mM BME