6OPD
Crystal Structure of ILNAMIVKI peptide bound to HLA-A2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 93 |
Detector technology | PIXEL |
Collection date | 2019-02-21 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 49.561, 74.641, 122.849 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 31.568 - 1.791 |
R-factor | 0.1673 |
Rwork | 0.166 |
R-free | 0.20430 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3pwl |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 31.568 | 1.836 |
High resolution limit [Å] | 1.791 | 1.791 |
Rpim | 0.037 | 0.213 |
Number of reflections | 42624 | 2553 |
<I/σ(I)> | 22.2 | |
Completeness [%] | 97.6 | |
Redundancy | 1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 20% PEG8000, 100 mM MES, pH 6.5, 200 mM magnesium acetate |