6OOM
PROTEIN A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-10-10 |
Detector | MAR CCD 130 mm |
Wavelength(s) | 1 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 95.239, 63.031, 102.340 |
Unit cell angles | 90.00, 101.28, 90.00 |
Refinement procedure
Resolution | 15.000 - 2.200 |
Structure solution method | SAD |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHARP |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 15.000 | 2.300 |
High resolution limit [Å] | 2.200 | 2.200 |
Number of reflections | 26746 | 300 |
<I/σ(I)> | 10 | |
Completeness [%] | 93.0 | |
Redundancy | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | PEG 400, magnesium chloride |