6OO0
Crystal structure of bovine Fab NC-Cow1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-03-23 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.0332 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 83.060, 69.560, 95.610 |
Unit cell angles | 90.00, 106.99, 90.00 |
Refinement procedure
Resolution | 28.865 - 2.100 |
R-factor | 0.2037 |
Rwork | 0.202 |
R-free | 0.23800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5ilt |
RMSD bond length | 0.002 |
RMSD bond angle | 0.583 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.900 | 2.150 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmeas | 0.162 | 1.460 |
Rpim | 0.078 | 0.693 |
Number of reflections | 30513 | 2243 |
<I/σ(I)> | 6 | 1 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 4.1 | 4.1 |
CC(1/2) | 0.991 | 0.303 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | 0.1M sodium cacodylate, 40% methylpentanediol, 5% PEG 8000 |