6ONW

Crystal structure of Sel1 repeat protein from Oxalobacter formigenes

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-12-06
Detector	DECTRIS PILATUS3 X 6M
Wavelength(s)	0.9791
Spacegroup name	P 21 21 21
Unit cell lengths	69.773, 74.079, 194.589
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.990 - 2.951
R-factor	0.2133
Rwork	0.211
R-free	0.25930
Structure solution method	SAD
RMSD bond length	0.002
RMSD bond angle	0.425
Data reduction software	HKL-3000
Data scaling software	SCALEPACK
Phasing software	HKL-3000
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.000
High resolution limit [Å]	2.950	8.000	2.950
Rmerge	0.169	0.063	0.730
Rmeas	0.189	0.071	0.833
Rpim	0.081	0.031	0.393
Number of reflections	21711	1215	992
<I/σ(I)>	4.3
Completeness [%]	98.7	98.6	92.8
Redundancy	5.2	4.9	3.9
CC(1/2)		0.991	0.597

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	289	0.1M Tris-Cl, 30% PEG1000