6ONT
Structure of the Francisella response regulator 1452 receiver domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-07-09 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.0332 |
Spacegroup name | I 41 3 2 |
Unit cell lengths | 128.726, 128.726, 128.726 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.512 - 1.803 |
R-factor | 0.1844 |
Rwork | 0.182 |
R-free | 0.20190 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5uic |
RMSD bond length | 0.005 |
RMSD bond angle | 0.782 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.830 |
High resolution limit [Å] | 1.800 | 1.800 |
Number of reflections | 17102 | |
<I/σ(I)> | 83.425 | 2.205 |
Completeness [%] | 100.0 | 99.94 |
Redundancy | 24.7 | 8 |
CC(1/2) | 0.083 | 0.502 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.2 M Calcium Chloride, 0.1 M HEPES pH 7.5, 28% PEG 400 |