6ONQ

Crystal structure of c-type cytochrome XoxG from Methylobacterium extorquens AM1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2018-08-30
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.6984
Spacegroup name	P 62 2 2
Unit cell lengths	95.841, 95.841, 80.411
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	41.500 - 2.710
R-factor	0.1963
Rwork	0.194
R-free	0.24840
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	0.970
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.760
High resolution limit [Å]	2.710	2.710
Rmerge	0.124	1.242
Rpim	0.020	0.256
Number of reflections	6337	305
<I/σ(I)>	39
Completeness [%]	98.6
Redundancy	37.7
CC(1/2)	0.999	0.898

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.2	298	.2 M sodium acetate, 0.1 M phosphate-citra te pH 4.2, and 20% (w/v) PEG 8000