6ONO
Complex structure of WhiB1 and region 4 of SigA in C2221 space group
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-12-02 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9793 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 37.445, 114.080, 144.713 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.790 - 1.850 |
| R-factor | 0.1967 |
| Rwork | 0.195 |
| R-free | 0.23700 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.871 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AutoSol |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.880 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.095 | 0.894 |
| Rmeas | 0.091 | 1.121 |
| Rpim | 0.018 | 0.258 |
| Number of reflections | 24510 | 736 |
| <I/σ(I)> | 36.8 | 0.84 |
| Completeness [%] | 91.5 | 56.4 |
| Redundancy | 22.1 | 13.2 |
| CC(1/2) | 0.998 | 0.822 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 291 | 100 mM Bis-Tris, pH 5.5, 200 mM magnesium chloride, 25% PEG3350 |
