6ONO
Complex structure of WhiB1 and region 4 of SigA in C2221 space group
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-12-02 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9793 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 37.445, 114.080, 144.713 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.790 - 1.850 |
R-factor | 0.1967 |
Rwork | 0.195 |
R-free | 0.23700 |
Structure solution method | SAD |
RMSD bond length | 0.006 |
RMSD bond angle | 0.871 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | AutoSol |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.880 |
High resolution limit [Å] | 1.850 | 1.850 |
Rmerge | 0.095 | 0.894 |
Rmeas | 0.091 | 1.121 |
Rpim | 0.018 | 0.258 |
Number of reflections | 24510 | 736 |
<I/σ(I)> | 36.8 | 0.84 |
Completeness [%] | 91.5 | 56.4 |
Redundancy | 22.1 | 13.2 |
CC(1/2) | 0.998 | 0.822 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 291 | 100 mM Bis-Tris, pH 5.5, 200 mM magnesium chloride, 25% PEG3350 |