6ONA
Crystal structure of Influenza hemagglutinin from strain A/Hickox/JY2/1940
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2019-03-21 |
Detector | RAYONIX MX-300 |
Wavelength(s) | 0.97872 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 108.560, 102.250, 160.950 |
Unit cell angles | 90.00, 91.07, 90.00 |
Refinement procedure
Resolution | 45.388 - 1.950 |
R-factor | 0.1936 |
Rwork | 0.193 |
R-free | 0.22970 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6mya |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (dev_3409) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 45.388 | 45.388 | 2.000 |
High resolution limit [Å] | 1.950 | 8.720 | 1.950 |
Rmerge | 0.077 | 0.026 | 0.875 |
Rmeas | 0.085 | 0.029 | 0.974 |
Total number of observations | 661978 | ||
Number of reflections | 127783 | 1497 | 9450 |
<I/σ(I)> | 12.85 | 38.59 | 2.13 |
Completeness [%] | 99.9 | 98.9 | 99.9 |
Redundancy | 5.18 | 4.884 | 5.301 |
CC(1/2) | 0.999 | 0.998 | 0.806 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 287 | 0.1 uL 9.5 mg/mL SEC-purified InvbQ.18715.a.KN11.PD38349 in 2 5 mM Tris, pH 8.5, 150 mM sodium chloride + 0.1 uL mother liquor (0.1 M sodium citrate, pH 5.5, 0.1 M magnesium chloride, 34% PEG400), flash-frozen, crystal ID 308018c7 data set les6-6 |