6ON9

Crystal Structure of the ZIG-8-RIG-5 IG1-IG1 heterodimer, tetragonal form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	120
Detector technology	PIXEL
Collection date	2019-02-27
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97920
Spacegroup name	P 41 21 2
Unit cell lengths	80.078, 80.078, 166.622
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	72.175 - 1.995
R-factor	0.1759
Rwork	0.174
R-free	0.20760
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6on6
RMSD bond length	0.018
RMSD bond angle	1.367
Data reduction software	XDS (20180808)
Data scaling software	XDS (20180808)
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX ((1.15rc3_3435: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	2.120
High resolution limit [Å]	1.995	2.000
Rmerge	0.178	1.693
Rmeas	0.185	1.764
Number of reflections	37606	5835
<I/σ(I)>	7.94	1.31
Completeness [%]	99.5	97.6
Redundancy	13.2	12.4
CC(1/2)	0.995	0.672

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	0.2 M disodium hydrogen phosphate, 20% PEG 3350