6ON7
Crystal Structure of Apo Domain-Swapped Dimer Q108K:T51D:A28C:L36C Mutant of Human Cellular Retinol Binding Protein II
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-10-13 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.97624 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 36.506, 60.197, 63.071 |
Unit cell angles | 90.00, 93.70, 90.00 |
Refinement procedure
Resolution | 31.470 - 1.981 |
R-factor | 0.2082 |
Rwork | 0.203 |
R-free | 0.25430 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2rct |
RMSD bond length | 0.008 |
RMSD bond angle | 0.951 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.14_3260)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 31.470 | 2.052 |
High resolution limit [Å] | 1.981 | 1.981 |
Rmerge | 0.090 | 0.417 |
Rmeas | 0.100 | 0.468 |
Number of reflections | 17686 | 1739 |
<I/σ(I)> | 18.2 | 2.81 |
Completeness [%] | 93.0 | 94.5 |
Redundancy | 4.9 | 4.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | PEG4000, ammonium acetate |