6ON7
Crystal Structure of Apo Domain-Swapped Dimer Q108K:T51D:A28C:L36C Mutant of Human Cellular Retinol Binding Protein II
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-10-13 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.97624 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 36.506, 60.197, 63.071 |
| Unit cell angles | 90.00, 93.70, 90.00 |
Refinement procedure
| Resolution | 31.470 - 1.981 |
| R-factor | 0.2082 |
| Rwork | 0.203 |
| R-free | 0.25430 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2rct |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.951 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.14_3260)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.470 | 2.052 |
| High resolution limit [Å] | 1.981 | 1.981 |
| Rmerge | 0.090 | 0.417 |
| Rmeas | 0.100 | 0.468 |
| Number of reflections | 17686 | 1739 |
| <I/σ(I)> | 18.2 | 2.81 |
| Completeness [%] | 93.0 | 94.5 |
| Redundancy | 4.9 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | PEG4000, ammonium acetate |
