6ON6

Crystal Structure of the RIG-5 IG1 homodimer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	120
Detector technology	PIXEL
Collection date	2018-05-22
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97946
Spacegroup name	P 61 2 2
Unit cell lengths	46.610, 46.610, 196.849
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.365 - 1.423
R-factor	0.1985
Rwork	0.197
R-free	0.22610
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6nrx
RMSD bond length	0.013
RMSD bond angle	1.302
Data reduction software	XDS (20180409)
Data scaling software	XDS (20180409)
Phasing software	PHASER (2.8.2)
Refinement software	PHENIX (1.15rc3_3435)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.510
High resolution limit [Å]	1.420	1.420
Rmerge	0.027	0.202
Rmeas	0.028	0.209
Number of reflections	24835	3827
<I/σ(I)>	55.51	9.46
Completeness [%]	99.6	97.7
Redundancy	17.7	15.2
CC(1/2)	1.000	0.996

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	295	0.1 M HEPES pH 7.0, 1 M Sodium citrate