6OLY

Full-length MthK channel at 3.1 angstrom resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	93
Detector technology	CCD
Collection date	2006-01-01
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	P 61 2 2
Unit cell lengths	137.456, 137.456, 373.026
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	39.702 - 3.112
R-factor	0.2376
Rwork	0.236
R-free	0.27460
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3rbz
RMSD bond length	0.006
RMSD bond angle	0.904
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((dev_3374: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.702	3.223
High resolution limit [Å]	3.112	3.112
Rmerge	0.134
Rpim	0.038
Number of reflections	37785	3251
<I/σ(I)>	25.4	1.9
Completeness [%]	98.2
Redundancy	11.6
CC(1/2)		0.489

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		300	28% PEG350-MME, 0.1M MES pH 6.5, 0.2 M CaCl2