6OLV

Protein Tyrosine Phosphatase 1B (1-301), P185A mutant, apo state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	93
Detector technology	CCD
Collection date	2016-07-25
Detector	RIGAKU SATURN 944
Wavelength(s)	1.54
Spacegroup name	P 31 2 1
Unit cell lengths	88.519, 88.519, 103.870
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	38.330 - 2.100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3qkq
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.330	2.175
High resolution limit [Å]	2.100	2.100
Rmerge	0.114	0.632
Rmeas	0.120	0.666
Rpim	0.036	0.207
Number of reflections	27591	2723
<I/σ(I)>	10.28
Completeness [%]	98.7	99.8
Redundancy	10.6	10

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	277	0.1M Hepes, 0.2 M magnesium acetate and 15-20% polyethylene glycol 8000