6OLM
CryoEM structure of PilT4 from Geobacter metallireducens with added ATP: C6cccccc conformation
EM specimen
| Vitrification | Yes |
EM Data Collection
| Microscope | FEI TECNAI 20 |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 200 |
| Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 4.4 |
| Software | cryoSPARC,PHENIX,UCSF Chimera |
| Symmetry | C6 |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
