6OK2
PilT4 from Geobacter metallireducens bound to ADP: C3ocococ conformation
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS-II BEAMLINE 17-ID-1 |
Synchrotron site | NSLS-II |
Beamline | 17-ID-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-07-11 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.9996 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 111.817, 120.956, 185.915 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.386 - 3.287 |
R-factor | 0.2174 |
Rwork | 0.215 |
R-free | 0.25550 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3jvv |
RMSD bond length | 0.003 |
RMSD bond angle | 0.577 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.386 | 3.400 |
High resolution limit [Å] | 3.287 | 3.287 |
Rpim | 0.069 | 0.501 |
Number of reflections | 38771 | 3597 |
<I/σ(I)> | 7.5 | |
Completeness [%] | 99.0 | |
Redundancy | 6.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.6 | 293 | 5.75 % (w/v) PEG3350 100 mM L-proline 50 mM Hepes pH 7.6 100 mM NaCl 25 mM Hepes pH 8.0 |