6OK1
Ltp2-ChsH2(DUF35) aldolase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08B1-1 |
| Synchrotron site | CLSI |
| Beamline | 08B1-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-05-25 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.9786 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 91.680, 110.560, 120.140 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.828 - 1.700 |
| R-factor | 0.1455 |
| Rwork | 0.144 |
| R-free | 0.16800 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.985 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | RESOLVE |
| Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.761 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.049 | |
| Number of reflections | 134309 | 11051 |
| <I/σ(I)> | 19.08 | 2.23 |
| Completeness [%] | 99.9 | 99.97 |
| Redundancy | 5.2 | 5.2 |
| CC(1/2) | 0.999 | 0.734 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 283 | 0.2 M sodium thiocyanate, 20% PEG 3350, 16 mg/ml protein |
