6OJY
Methylated PilT4 from Geobacter metallireducens bound to sulfate: C3ocococ conformation
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-12-16 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.9795 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 115.800, 119.010, 178.300 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.558 - 3.300 |
R-factor | 0.2107 |
Rwork | 0.209 |
R-free | 0.24600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3jvv |
RMSD bond length | 0.005 |
RMSD bond angle | 0.894 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.558 | 3.400 |
High resolution limit [Å] | 3.300 | 3.300 |
Rpim | 0.067 | 0.400 |
Number of reflections | 37753 | 3658 |
<I/σ(I)> | 12.6 | |
Completeness [%] | 100.0 | |
Redundancy | 7.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 25 mM Hepes pH 7 75 mM NaCl 5 % (v/v) glycerol 100 mM ammonium sulphate 50 mM Bis-Tris-HCl pH 6.5 12.5% (w/v) PEG3350 |