6OJX

PilT4 from Geobacter metallireducens bound to ATP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-04-18
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0332
Spacegroup name	P 6
Unit cell lengths	190.187, 190.187, 60.455
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.547 - 1.892
R-factor	0.1846
Rwork	0.184
R-free	0.21240
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3jvv
RMSD bond length	0.002
RMSD bond angle	0.535
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.14_3211: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.000	2.000
High resolution limit [Å]	1.892	1.892
Number of reflections	99891	9942
<I/σ(I)>	10.6
Completeness [%]	100.0
Redundancy	10.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	293	1mM ATP 1mM MgCl2 4.5 % (w/v) PEG4000 50 mM MES pH 6 100 mM MgCl2 100 mM NaCl 25 mM Hepes pH 8