6OJX
PilT4 from Geobacter metallireducens bound to ATP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-04-18 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.0332 |
Spacegroup name | P 6 |
Unit cell lengths | 190.187, 190.187, 60.455 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 47.547 - 1.892 |
R-factor | 0.1846 |
Rwork | 0.184 |
R-free | 0.21240 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3jvv |
RMSD bond length | 0.002 |
RMSD bond angle | 0.535 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((1.14_3211: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.000 | 2.000 |
High resolution limit [Å] | 1.892 | 1.892 |
Number of reflections | 99891 | 9942 |
<I/σ(I)> | 10.6 | |
Completeness [%] | 100.0 | |
Redundancy | 10.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 1mM ATP 1mM MgCl2 4.5 % (w/v) PEG4000 50 mM MES pH 6 100 mM MgCl2 100 mM NaCl 25 mM Hepes pH 8 |