6OJK
Structure of YePL2A K291W in Complex with Tetragalacturonic Acid
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-10-03 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.97949 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 58.240, 94.930, 126.770 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.224 - 1.500 |
| R-factor | 0.174 |
| Rwork | 0.173 |
| R-free | 0.18760 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2v8k |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.830 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.250 | 1.517 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.044 | 0.450 |
| Number of reflections | 112653 | 3490 |
| <I/σ(I)> | 17.27 | 2.56 |
| Completeness [%] | 99.7 | 99.96 |
| Redundancy | 4.5 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | PEG 1500, Sodium Acetate |
