6OJJ
Structure of ScAtg3 with truncations in N-terminal and flexible region (FR)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-02-23 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 92.078, 44.557, 66.200 |
| Unit cell angles | 90.00, 102.98, 90.00 |
Refinement procedure
| Resolution | 26.408 - 2.406 |
| R-factor | 0.2146 |
| Rwork | 0.213 |
| R-free | 0.24320 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2dyt |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.674 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.440 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rpim | 0.094 | 0.527 |
| Number of reflections | 10013 | 482 |
| <I/σ(I)> | 12.4 | 1.3 |
| Completeness [%] | 97.9 | 92.7 |
| Redundancy | 3.4 | 2.8 |
| CC(1/2) | 0.587 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 277.15 | 9% PEG3350, 100 mM HEPES pH 7.2, 150 mM L-Malic acid. |
