6OJ1
Crystal Structure of Aspergillus fumigatus Ega3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08B1-1 |
Synchrotron site | CLSI |
Beamline | 08B1-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-02-19 |
Detector | RAYONIX MX300HE |
Wavelength(s) | 0.97947 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 44.640, 47.310, 152.510 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.186 - 1.760 |
R-factor | 0.1634 |
Rwork | 0.162 |
R-free | 0.19190 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2aam |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | Arcimboldo (Shredder) |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.186 | 1.820 |
High resolution limit [Å] | 1.760 | 1.760 |
Rmerge | 0.063 | 0.595 |
Rmeas | 0.066 | 0.620 |
Rpim | 0.017 | 0.180 |
Number of reflections | 32553 | 2863 |
<I/σ(I)> | 28.1 | 4.27 |
Completeness [%] | 99.8 | 88.7 |
Redundancy | 14 | 11.4 |
CC(1/2) | 1.000 | 0.917 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2 M potassium iodide, 20 % (v/v) PEG 3350 |