6OID
Redox Regulation of FN3K from Arabidopsis thaliana
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-11-30 |
Detector | MARRESEARCH |
Wavelength(s) | 1.0 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 158.860, 163.640, 70.720 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 53.504 - 2.365 |
R-factor | 0.1765 |
Rwork | 0.175 |
R-free | 0.20400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3f7w 3jr1 and 5IGS |
RMSD bond length | 0.009 |
RMSD bond angle | 1.071 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 53.504 | 2.430 |
High resolution limit [Å] | 2.350 | 2.370 |
Rmeas | 0.157 | |
Number of reflections | 38000 | 2683 |
<I/σ(I)> | 14.3 | |
Completeness [%] | 99.8 | 97.1 |
Redundancy | 14.5 | 14.6 |
CC(1/2) | 0.999 | 0.520 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | 2 M Ammonium sulfate, 0.1 M MES pH 5.5 |