6OI0
Crystal structure of human WDR5 in complex with L-arginine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-10-24 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 0.97931 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 81.186, 86.703, 40.963 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 59.260 - 1.920 |
| R-factor | 0.16203 |
| Rwork | 0.160 |
| R-free | 0.20068 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2h14 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.449 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 59.260 | 1.970 |
| High resolution limit [Å] | 1.920 | 1.920 |
| Rpim | 0.040 | 0.290 |
| Number of reflections | 22592 | 1272 |
| <I/σ(I)> | 10.7 | 3.1 |
| Completeness [%] | 99.0 | 84.7 |
| Redundancy | 12.5 | 10.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.9 | 295 | 100 mM Bis-Tris pH 5.9; 32% PEG3350, 54.6 mM Ammonium Sulfate, 0.66 mM L-arginine |
