6OHM

Structure of tungstate bound human Phospholipase D2 catalytic domain

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-08-27
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	1.2
Spacegroup name	C 1 2 1
Unit cell lengths	92.515, 132.242, 107.089
Unit cell angles	90.00, 111.45, 90.00

Refinement procedure

Resolution	48.550 - 1.895
R-factor	0.1835
Rwork	0.182
R-free	0.20570
Structure solution method	SAD
RMSD bond length	0.003
RMSD bond angle	0.608
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.960
High resolution limit [Å]	1.895	1.895
Number of reflections	94443	9136
<I/σ(I)>	12.2
Completeness [%]	100.0
Redundancy	6.7
CC(1/2)	0.990

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.7	298	0.2M ammonium sulfate, 20% PEG3350, 0.1M bis-tris pH 6.7