6OHI
Crystal Structure of the Debrominase Bmp8 (Apo)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-04-21 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.00 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 65.459, 65.459, 288.707 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 56.690 - 2.270 |
R-factor | 0.19952 |
Rwork | 0.198 |
R-free | 0.23538 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.005 |
RMSD bond angle | 0.981 |
Data reduction software | XDS |
Data scaling software | autoPROC |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 144.400 | 2.312 |
High resolution limit [Å] | 2.270 | 2.272 |
Rmerge | 1.522 | |
Rpim | 0.032 | 0.317 |
Number of reflections | 17455 | 873 |
<I/σ(I)> | 17.1 | 2.2 |
Completeness [%] | 97.8 | 100 |
Redundancy | 21.9 | 23.7 |
CC(1/2) | 0.990 | 0.801 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9 | 282 | 2.3 M ammonium sulfate, 0.1 M Bistris propane pH 9.0), 7 mg/mL Bmp8 |