6OHG
Structure of Plasmodium falciparum vaccine candidate Pfs230D1M in complex with the Fab of a transmission blocking antibody
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-06-11 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 79.954, 191.334, 41.198 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 24.049 - 2.385 |
R-factor | 0.233 |
Rwork | 0.229 |
R-free | 0.27710 |
Structure solution method | MOLECULAR REPLACEMENT |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 24.049 | 24.049 | 2.530 |
High resolution limit [Å] | 2.385 | 6.890 | 2.390 |
Rmerge | 0.051 | 0.031 | 0.321 |
Rmeas | 0.057 | 0.035 | 0.363 |
Number of reflections | 25684 | 1165 | 3756 |
<I/σ(I)> | 17.16 | 33.1 | 3.86 |
Completeness [%] | 98.3 | 96.1 | 91.9 |
Redundancy | 4.663 | 4.184 | 4.41 |
CC(1/2) | 0.999 | 0.999 | 0.957 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | 15-22% PEG 4000, sodium acetate pH=4.6, 0.05-0.1 molar magnesium chloride, 20% glycerol |