6OHB

E. coli Guanine Deaminase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-06-23
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9198
Spacegroup name	P 1 21 1
Unit cell lengths	66.479, 137.583, 98.581
Unit cell angles	90.00, 102.16, 90.00

Refinement procedure

Resolution	55.990 - 2.300
R-factor	0.188
Rwork	0.186
R-free	0.23430
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ood
RMSD bond length	0.004
RMSD bond angle	0.796
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	55.990	2.350
High resolution limit [Å]	2.300	2.300
Rmerge	0.061	0.550
Number of reflections	73766	4626
<I/σ(I)>	8.3
Completeness [%]	96.6
Redundancy	2.4
CC(1/2)	0.989	0.731

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	20% Peg3350 100 mM Bis-tris propane pH 7.6 30 mM Citric acid