6OHA
Yeast Guanine Deaminase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS-II BEAMLINE 17-ID-1 |
Synchrotron site | NSLS-II |
Beamline | 17-ID-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-08-15 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.9201 |
Spacegroup name | P 43 2 2 |
Unit cell lengths | 106.812, 106.812, 114.178 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.070 - 2.210 |
R-factor | 0.1584 |
Rwork | 0.156 |
R-free | 0.20340 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2ood |
RMSD bond length | 0.012 |
RMSD bond angle | 1.139 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.070 | 2.290 |
High resolution limit [Å] | 2.210 | 2.210 |
Rmerge | 0.146 | 0.973 |
Number of reflections | 33428 | 3075 |
<I/σ(I)> | 10 | |
Completeness [%] | 99.1 | |
Redundancy | 8.8 | |
CC(1/2) | 0.996 | 0.696 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 17% Peg 3350 0.2 M ammonium sulfate 100 mM Hepes, pH 7.5 |