6OH7

Crystal structure of (E)-biformene synthase LrdC from Streptomyces sp. strain K155 in complex with Mg

Replaces:  5A0K

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-BM
Synchrotron site	APS
Beamline	19-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2014-11-18
Detector	ADSC QUANTUM 210r
Wavelength(s)	0.9790
Spacegroup name	P 32 2 1
Unit cell lengths	107.050, 107.050, 89.241
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	32.616 - 2.190
R-factor	0.2357
Rwork	0.234
R-free	0.26120
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	LOW RESOLUTION SAD STRUCTURE
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.270	2.260
High resolution limit [Å]	2.190	2.190
Rmerge	0.092	1.230
Number of reflections	30779	2617
<I/σ(I)>	18.2	2
Completeness [%]	100.0	100
Redundancy	14.3	12.5
CC(1/2)	0.999	0.726

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	6.5	277	0.16 M MAGNESIUM ACETATE TETRAHYDRATE, 0.08 M SODIUM CACODYLATE TRIHYDRATE PH 6.5, 16% (W/V) PEG 8000, 20% (V/V) GLYCEROL