6OH6

Crystal structure of (E)-biformene synthase LrdC from Streptomyces sp. strain K155 in complex with Mg and PPi

Replaces:  5A0J

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-09-27
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.1000
Spacegroup name	P 32 2 1
Unit cell lengths	107.050, 107.050, 88.821
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.354 - 2.070
R-factor	0.2344
Rwork	0.233
R-free	0.25250
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	LOW RESOLUTION SAD STRUCTURE
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.354	2.130
High resolution limit [Å]	2.070	2.070
Rmerge	0.071	1.194
Number of reflections	36222	2766
<I/σ(I)>	22	2
Completeness [%]	100.0	99.9
Redundancy	17.1	10
CC(1/2)	0.999	0.643

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	6.5	277	0.16 M MAGNESIUM ACETATE TETRAHYDRATE, 0.08 M SODIUM CACODYLATE TRIHYDRATE PH 6.5, 16% (W/V) PEG 8000, 20% (V/V) GLYCEROL