6OGC

Crystal structure of Green Fluorescent Protein (GFP); S65T, Y66(2,3-F2Y), H148D; circular permutant (50-51)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-11-21
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9795
Spacegroup name	P 1 21 1
Unit cell lengths	52.275, 68.866, 60.968
Unit cell angles	90.00, 100.34, 90.00

Refinement procedure

Resolution	36.500 - 1.178
R-factor	0.1443
Rwork	0.143
R-free	0.16090
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4zf3
RMSD bond length	0.014
RMSD bond angle	1.397
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((1.13rc2_2986: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.500	1.221
High resolution limit [Å]	1.178	1.178
Rmerge	0.048	0.551
Rmeas	0.050	0.580
Rpim	0.014	0.173
Number of reflections	134742	11199
<I/σ(I)>	29.19	3.65
Completeness [%]	95.0	80.44
Redundancy	13.1	10.5
CC(1/2)	1.000	0.905

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	295	50 mM sodium acetate, pH 5.0, 100 mM NaCl, 7-8% PEG 3350