6OGA
Crystal structure of Green Fluorescent Protein (GFP); S65T, Y66(3-Br1Y), H148D; circular permutant (50-51)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-11-02 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9795 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 51.600, 68.600, 61.450 |
Unit cell angles | 90.00, 99.82, 90.00 |
Refinement procedure
Resolution | 34.300 - 1.600 |
R-factor | 0.1625 |
Rwork | 0.161 |
R-free | 0.18620 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4zf3 |
RMSD bond length | 0.007 |
RMSD bond angle | 0.866 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 34.300 | 1.657 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.074 | 1.092 |
Rmeas | 0.081 | 1.199 |
Rpim | 0.033 | 0.488 |
Number of reflections | 54833 | 5471 |
<I/σ(I)> | 13.38 | 1.38 |
Completeness [%] | 98.3 | 98.75 |
Redundancy | 5.7 | 5.8 |
CC(1/2) | 0.999 | 0.592 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 295 | 50 mM sodium acetate, pH 5.0, 100 mM NaCl, 7-8% PEG 3350 |